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Hello.

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My name is Ryan Kennedy and I'm a product
manager here at Sysmex America.

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Today, I have the pleasure
of demonstrating for you

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some of the Gating software

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capabilities on the Sysmex instrument
for animal testing laboratories.

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The XN-V automated hematology analyzer.
One of the fantastic features

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of the XN-V Series analyzer is the ability
to electronically re-analyze

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a sample’s data under a different species
algorithm calculation,

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which is going to reclassify the Gates
and generate any new flagging

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and results if necessary
based on that new species selection

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without needing to aspirate
any additional sample.

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To dive a little deeper
into this functionality,

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we're going to go over to the Species
Settings application within our analyzer.

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On the main menu of the XN-V,
you'll notice that there's an icon

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for species setting.

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This is the area of the analyzer

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where you can customize any of the species
that are built into the analyzer,

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as well as modify or create
entirely new sample judgment criteria.

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When you go into the setting, it's
going to open up a pop up window.

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Now I just want to touch on each one
of these selections briefly.

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The ‘Manage Settings’

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is where you can download or upload

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different species
Gates that you've already set.

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The XN-V solution comes preloaded
with multiple species already classified

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for the different
Gating of the populations,

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for the various measurement channels
available on the analyzer.

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However, say hypothetically,
you have a research group

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that you're performing testing on
and you modify those Gates somewhat

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and now you have a sister site
that is doing a similar research study.

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Those Gates that you modified can be
downloaded and sent to that sister site

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so you can share information
extremely easily

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to any other laboratories
for collaboration purposes.

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‘Name/Comment/Analysis’

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This is where you're going to see
all your different species types

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that come prebuilt with the analyzer

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and you will also see
any new ones that you've built as well.

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There are a lot of different customization
options available,

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but just to summarize,
you can build subspecies off of an already

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existing species type

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make entirely new species
to start building Gates with them,

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as well as make modifications
on how they analyze the reports out; WBC,

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Platelet, Hemoglobin and Hematocrit

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parameters for each of the species types.

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While we're on the screen,
I also want to point out that on your

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XN-V Series instrument,
you have two different fluorescent flow

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cytometry methodologies available 
to calculate your WBC parameter.

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In addition to having 
three different methodologies

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available
to calculate the platelet parameter,

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this gives you parameter 
measurement redundancy in cases

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where you have abnormal pathologies,
and having those additional automated

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measurement options
greatly reduces the likelihood

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of needing to go to these time
extensive and less precise manual methods.

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‘Reference Interval’ this is something
that you could use or not use,

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but you'll have the capability
if you find it useful in your laboratory.

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If you do decide to use it,
you would just go into this screen here

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and select your different species
and indicate your lower and upper limits.

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So on your printouts or on your explorer
screen, you would see whether or not

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the results were high or low
or within your expected range

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‘List Order’

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this is where you can customize
your list of species for your laboratory.

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So, for example, if you don't analyze

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horse, rabbit or cattle specimens,
you can remove them from the selected

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items on the right so that your scientists
don't select them by mistake.

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Or if you just want to clean up the list
so that you aren't scrolling

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through a bunch of different

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species types that you never use
to get to the ones you actually do.

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Again...

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Another feature in here to customize
and make it the most user friendly to you.

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Flagging capabilities on this instrument
are completely customizable

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and can be tailored specifically

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for any of the species profiles
that you have built on your instrument.

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In this area you can see that
you have multiple different flagging

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criteria available for your WBC,
RBC and platelet parameters.

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And all you would need to do is select
which species type

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you want to adjust flagging criteria on

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and then which flags you want
to have populated on your analyzer.

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And for those flags you want to populate,
you would indicate

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what cut off limits
you want for each of those flags.

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Earlier we mentioned the ability
to electronically re-analyze

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a sample's data under a different species
algorithm calculation

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which allows you to on the fly
make incredibly quick changes to results

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based on species profiles
that already exist within your instrument.

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Well, now let's take a look at 
the ability to adjust the actual gates

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on the individual species
within the manual analysis section.

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First, let's go to our sample explorer,

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then data browser section.

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Then let's select ‘ReAna’ on a specimen

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that we want to modify

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and then select ‘Manual Analysis’

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This is going to open up a new window
where you have your sample demographic

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information at the top

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and your sample results

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over on the left hand side.

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You have your different gate sections
over here on the right hand side.

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So you can see that each of
the fluorescent flow cytometry measurement

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channels, as well as the impedance
channels, are available for customization.

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Now let's look at the WNR fluorescent

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flow cytometry measurement channel.

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Which is normally capturing nRBC, Basophil

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and a total white
blood cell count parameter.

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And let's say that you feel that

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this isn't capturing
all of the three parameters correctly.

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Simply select the different groupings
that are preset here,

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then left click
to generate a different vertex.

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And then when you want to close the gate,
you would right click

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and then just continue 
to do that for each gate.

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Also, you could type in over here
the specific x and y coordinates

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if you feel more comfortable
with that option or to make minor tweaks.

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And then once you hit reanalyze,
you will see the information populate over

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on the left hand side of the screen
to the right of the original results list.

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So now you can see the two results
based on the different gatings

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and how they compare and then select
which one is going to work for you.

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Additionally, if you feel that
this new gating

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that you have created is something
you want to use moving forward,

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you can select at the bottom here
‘Gate Save’

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and save that information
to be available for use on future samples.

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Or if you feel that you aren't 
satisfied with the gates

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that you've made,
you can discard by selecting exit.

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So the last feature of the re-analysis
menu functionality that we're going to

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briefly go over is the ability to create
new gates entirely.

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Which you can perform
under the manual analysis ‘EXT.’

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or extended.

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This area is going to look very similar
to the previous selection,

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that simple manual analysis area.

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However, this is specific
just to those fluorescence flow

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cytometry measurement channels
and their associated parameters.

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And you can see here
that you can very easily create new gates

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within any of those fluorescent flow
cytometry measurement channels,

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again, simply by using your mouse
with the left clicks, generating

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each vertex and right clicking
when you want to close the loop

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or by typing in
specific x and y coordinates.

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This area within the analyzer

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allows you to go beyond what's 
already pre built on the system.

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If your laboratory so desires
for your research purposes.

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For each channel,
you can create up to seven

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individual gates with an eight gate
being the total enumeration

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of subsequent gates
within its specified region limits.

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In addition to our innovative technology
with the XN-V Series Solutions,

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you will also gain the ability
to vastly customize your animal testing.

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As one of the leaders in hematology
laboratory diagnostics, Sysmex remains

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committed to supporting veterinary testing
facilities, your unique needs

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and the vital role that you play in the
health care and pharmaceutical industry.