Fluorescent Platelet (PLT-F) Application

Fluorescent Platelet (PLT-F) Application

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The fluorescent platelet (PLT-F) channel on Sysmex’s XN-Series™ analyzers provides accurate and precise platelet counts, even in thrombocytopenic patients.

Have confidence in your PLT results

  • LT-F provides high measurement accuracy in the low concentration range.
  • Utilizing flow cytometry technology, the fluorescent stain specifically labels platelets, minimizing interference from WBC and RBC fragments.
  • In scientific research articles, PLT-F was found to correlate highly with the reference method CD41/CD61*.

 

Immature platelet assessment adds value

Leveraging the use of flow cytometry and specialized stains, the PLT-F channel also provides information that can help clinicians assess platelet production. The immature platelet fraction (IPF), one of Sysmex’s advanced parameters, identifies and enumerates platelets recently released by the bone marrow.

  • Reported as a relative (%) or absolute (#) result
  • Provides a more direct measurement of immature platelets compared to the mean platelet volume (MPV)
  • Increased IPF result correlates with increased circulation of immature platelets, indicating increased production of platelets in the bone marrow

 

The IPF serves as an excellent screening tool that helps to determine the etiology of thrombocytopenia by evaluating the current status of platelet production. In patients with low platelet counts, the bone marrow should increase platelet production, leading to an elevated immature platelet fraction. In some cases of thrombocytopenia, the bone marrow is unable to generate new platelets, resulting in a low or normal IPF.

Measurement technology

In the PLT-F channel, the membranes of the platelets are perforated, allowing the fluorescent stain (Fluorocell™ PLT) to bind to the intracellular RNA. The sample is then counted in the flow cell utilizing five times the volume that is used in the primary platelet count (PLT-I), making the PLT-F and IPF counts highly precise, even in patients with thrombocytopenia.

  • Platelet-specific stain minimizes interference with WBC or RBC fragments.
  • PLT-F shows excellent correlation with CD41/CD61 reference method*.
  • The immature platelet fraction enumerates the platelets most recently produced in bone marrow.

 

Workflow automation

For samples with platelet flags due to very low counts or potential interference in the impedance channel, the analyzer automatically reflexes measurement in the dedicated PLT-F channel.

  • PLT-F is a reliable, secondary platelet count reflexed automatically from onboard rules or Caresphere™ Workflow Solution software.
  • PLT-F is only used when required—saving time and cost by automatically retesting thrombocytopenic samples and those with unreliable impedance counts.
  • In patients with unclear thrombocytopenia, PLT-F analysis with IPF provides rapid, reliable and relevant clinical information.

 

*References

Tanaka Y, Tanaka Y, Gondo K, Maruki Y, Kondo T, Asai S, Matsushita H, Miyachi H. Performance evaluation of platelet counting by novel fluorescent dye staining in the XN-series automated hematology analyzers. J Clin Lab Anal. 2014 Sep;28(5):341-8. doi: 10.1002/jcla.21691. Epub 2014 Mar 19. PMID: 24648166; PMCID: PMC6807536

Wada A, Takagi Y, Kono M, Morikawa T (2015) Accuracy of a New Platelet Count System (PLT-F) Depends on the Staining Property of Its Reagents. PLOS ONE 10(10): e0141311. https://doi.org/10.1371/journal.pone.0141311


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