Hematology - More than just a CBC: Automated NRBC counts with every result
Hematology - More than just a CBC: Automated NRBC counts with every result
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Neonatology
Ideal for high-risk premature infants or pediatric patients. The CBC with NRBC count on every sample ensures
NRBCs are detected, even in low numbers. Through the use of flow cytometry, accurate WBC counts are ensured
regardless of NRBC concentration.
Intensive care units
Critically ill patients require careful monitoring. Published research studies show that a routine NRBC count with
every CBC enables early recognition of critical developments1, even at low NRBC concentrations.
Lab Workflow Benefits
- Because the NRBC count is routinely included in all measurement profiles, reflex testing for NRBCs is no longer necessary. This eliminates the need for additional testing and can therefore improve turnaround time.
- NRBCs and white blood cells are counted separately, so there is no need to correct the WBC count for the presence of NRBCs. Accurate white blood cell counts save time spent on smear preparation and manual counting, particularly during neonatal blood analysis.
- As a result, workflow is both accelerated and standardized. This reduces potential reporting errors and
enables critical results to be tracked over time.
Diagnostic Parameters
- WBC, NRBC%, NRBC#, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-SD, RDW-CV, PLT, PDW, MPV
- With every CBC measurement, the analyzer counts the nucleated red blood cells. Even when NRBCs are present, they do not interfere with the white blood cell count.
Technology of WBC and NRBC detection
First, the cell membrane of the white blood cells is perforated, during which time the
cells remain intact. The intracellular nucleic acids are then labelled with a specific
fluorescent marker. For NRBCs, the cell membrane is completely lysed and only the
nucleus is labelled.
The sample is then analyzed using fluorescent flow cytometry and measurement
signals related to forward scatter (FSC), side scatter (SSC) and side fluorescence (SFL).
Algorithms in the WNR scattergram identify WBC and NRBCs populations. The flexible
gating algorithm considers biological variance when evaluating measured signals.
Technology of RBC and PLT detection
Impedance counting technology utilizing hydrodynamic focusing increases reliability of results. Application of a sheath fluid allows the cells to pass through the detector unit individually and centrally, providing consistent and accurate cell counts.
Cyanide-free SLS hemoglobin measurement method
The dedicated sodium lauryl sulfate reagent lyses RBCs, WBCs and lipids equally and, due to the reduction in
potential interferences, delivers reliable HGB results.
Cumulative pulse height hematocrit measurement
This technology provides a direct hematocrit measurement that can be retraced to the reference method.
Measurement modes
Whole blood mode: the standard mode with an aspiration volume of only 88 μL of blood.
Pre-diluted mode: for capillary blood samples, only 20 μL of blood are required.
1 Menk M, et al. Nucleated red blood cells as predictors of mortality in patients with acute respiratory distress syndrome (ARDS): an observational study. Ann Intensive Care. 2018 Mar 27;8(1):42. doi: 10.1186/s13613-018-0387-5. PMID: 29589209; PMCID: PMC5869325.
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