Automated Differential Application

Automated Differential Application

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An automated differential that is efficient and advanced

The automated differential channel on the Sysmex XN-Series™ and XN-L Series™ analyzers is designed to provide accurate and reliable results through advanced technology. Benefits of utilizing the Sysmex automated differential include:

  • Six WBC differential parameters including automated Immature Granulocytes
  • Highly sensitive and standardized differentials, regardless of user experience
  • Special ‘Low WBC’ mode for obtaining accurate differentials on critically low cell counts

Sensitively detect abnormal blood count results

The advanced algorithms and reliable flagging messages alert users to potential white blood cell abnormalities. With high sensitivity, the Sysmex Adaptive Cluster Analysis System (ACAS) recognizes the shape of subpopulation clusters in the scattergrams, and delivers additional information to support diagnosis.

Your benefits in daily routine

  • Parameters: NEUT%, NEUT#, LYMPH%, LYMPH#, MONO%, MONO#, EO%, EO#, BASO%, BASO#, IG%, IG#
  • The DIFF channel provides reliable flagging of suspected malignant and reactive cells for further investigation.
  • A comprehensive range of parameters and messages may provide valuable information to support the diagnosis and monitoring of patient conditions.
  • The Immature Granulocyte (IG) parameter enables many laboratories to significantly reduce the number of manual smear reviews by implementing individual threshold values.*
  • A ‘Low WBC’ mode can be implemented when the WBC count is critically low and a neutrophil count cannot be obtained. By increasing the counting volume, the reliability of the results increases for all WBC differentiation parameters.

 * Immature Granulocytes are Key to Workflow Optimization

Technology of WBC differentiation

The specially developed lysis reagent (Lysercell™ WDF) perforates the cell membranes while leaving the cells largely intact. The fluorescent stain (Fluorocell™ WDF) then enters the cell and labels the intracellular nucleic acids. Cells are differentiated in the flow cell according to their fluorescence signal, size and internal structure. The intensity of the fluorescence signal is directly affected by the nucleic acid content and membrane composition of the cell. Some of the strongest fluorescence signals are shown by immature and activated cells.

Measurement mode

Whole blood mode: the standard mode with an aspiration volume of only 88 µL of blood. Low WBC mode: If an automated differential cannot be obtained due to leukocytopenia, samples can be re-measured in the LWBC mode as an automatic reflex test. The aspiration volume in this mode is 88 µL of whole blood.

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